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Rapid HPLC determination of g-aminobutyric acid and some other amino acids: application to rat brain
Summary
Type:
HPLC
Application #:
107700
Catalog#:
720120.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x150mm
Detection:
fluorescence, excitation 333nm, emission 532nm
Conditions:
eluent: water - acetonitrile (72:28, v/v), pH adjusted to 3.0 with 5M H3PO4
flow rate 1 ml/min
Authors:
Cann-Moisan C
Source:
J Chromatogr 532 (1990) 438 - 441
Details
Legend: Fig. 1. Chromatogram of a standard mixture (25 ng injected), a) diluted in NaHCO3, b) diluted in mobile phase, c) extract from rat brain, 1. Gln, 2. Ala, 3. GABA, 4. d-aminovaleric acid (int. std.).
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